Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca 2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones. Constructing a patch clamp setup. Heterologous Expression Systems and Neurones. The use of quartz patch pipettes for low noise single channel recording. On the basis of these electrical characteristics it is expected that patch pipettes pulled from quartz tubing will produce significantly less noise than pipettes made from other glasses. Our work confirms these expectations and we describe theoretical and practical aspects of the use of quartz pipettes for single channel patch voltage clamp measurements. Methods for pulling quartz pipettes with a laser- based puller and coating them with low- loss elastomers are discussed, as are precautions that are necessary to achieve low noise recordings. We have shown that quartz pipettes can be pulled from tubing with outer diameter to inner diameter ratios as large as 3 and a method of applying heavy elastomer coatings all the way to the tip of pipettes is presented. Noise sources arising from the pipette and its holder are described theoretically, and it is shown that measured noise is in good agreement with such predictions. With low noise capacitive feedback electronics, small geometry holders, and thick- walled quartz pipettes coated with low- loss elastomers we have been routinely able to achieve noise of 1. A rms or less in a 5- k. Hz bandwidth with real cell patches and a pipette immersion depth of approximately 2 mm. On occasion we have achieved noise as low as 6.
A rms in this bandwidth. Patch-Clamp Techniques Applied To Brain Slices By: James R. Advances in Physiology Education Jun 2009, 33 (2) 121-129; DOI: 10.1152/advan.90205.2008.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
December 2016
Categories |